Language
English
Publication Date
5-1-2025
Journal
Nature Methods
DOI
10.1038/s41592-025-02664-9
PMID
40263584
PMCID
PMC12074994
PubMedCentral® Posted Date
4-22-2025
PubMedCentral® Full Text Version
Post-print
Abstract
The spatial distribution of diverse biomolecules in multicellular organisms is essential for their physiological functions. High-throughput in situ mapping of biomolecules is crucial for both basic and medical research, and requires high scanning speed, spatial resolution, and chemical sensitivity. Here we developed a tissue-expansion method compatible with matrix-assisted laser desorption/ionization mass-spectrometry imaging (TEMI). TEMI reaches single-cell spatial resolution without sacrificing voxel throughput and enables the profiling of hundreds of biomolecules, including lipids, metabolites, peptides (proteins), and N-glycans. Using TEMI, we mapped the spatial distribution of biomolecules across various mammalian tissues and uncovered metabolic heterogeneity in tumors. TEMI can be easily adapted and broadly applied in biological and medical research, to advance spatial multi-omics profiling.
Keywords
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Animals, Mice, Humans, Single-Cell Analysis, Metabolomics, Mass spectrometry, Molecular imaging, Lipidomics, Lipidomics
Published Open-Access
yes
Recommended Citation
Zhang, Hua; Ding, Lang; Hu, Amy; et al., "TEMI: Tissue-Expansion Mass-Spectrometry Imaging" (2025). Faculty, Staff and Students Publications. 6472.
https://digitalcommons.library.tmc.edu/baylor_docs/6472