Language
English
Publication Date
1-1-2023
Journal
Methods in Molecular Biology
DOI
10.1007/978-1-0716-2760-0_12
PMID
36331770
Abstract
West Nile virus (WNV) is one of the leading causes of arboviral encephalitis in the United States but is often underdiagnosed. Despite the wide breadth of WNV-induced clinical disease syndromes, many of the symptoms associated with WNV are nonspecific at the time of presentation; thus, choosing the right diagnostic tool is essential to not only understand the true burden of disease but also provide pathogen-directed interventions for WNV-infected patients. In this chapter, we briefly discuss the three most common types of diagnostic methods for WNV in human clinical samples: nucleic acid detection, enzyme-linked immunoassay (ELISA), and plaque reduction neutralization test (PRNT) and present the method for PRNT.
Keywords
Humans, West Nile virus, Antibodies, Viral, Enzyme-Linked Immunosorbent Assay, West Nile Fever, Diagnostics, Flavivirus, PRNT, West Nile virus
Published Open-Access
yes
Recommended Citation
Nwanosike, Hephzibah; Green, Freedom M; Murray, Kristy O; et al., "Protocol of Detection of West Nile Virus in Clinical Samples" (2023). Faculty, Staff and Students Publications. 6504.
https://digitalcommons.library.tmc.edu/baylor_docs/6504