Language

English

Publication Date

6-25-2025

Journal

Journal of the American Chemical Society

DOI

10.1021/jacs.4c17876

PMID

40493376

PMCID

PMC12203648

PubMedCentral® Posted Date

6-27-2025

PubMedCentral® Full Text Version

Author MSS

Abstract

Antibiotics are essential for modern medicine, but their use drives the evolution of antimicrobial resistance (AMR) that limits the long-term efficacy of any one drug. To keep pace with AMR and preserve our ability to treat bacterial infections, it is essential that we identify antibiotics with new structures and targets that are not affected by clinical resistance. Historically, most developmental candidates for antibiotics have come from microbial natural products, as they feature chemical structures and biological activities that have been honed over millions of years of evolution. Unfortunately, as classical bioactivity screens for natural product discovery are blind to the pharmacological properties of their hits, they often identify molecules with functional groups that limit their utility as drugs. One prominent example is actinonin, an inhibitor of bacterial peptide deformylase (PDF) whose activity is dependent on a hydroxamate moiety associated with toxicity

Keywords

Amidohydrolases, Biological Products, Anti-Bacterial Agents, Enzyme Inhibitors, Genome, Bacterial, Hydroxamic Acids, Microbial natural products, genome-mining, biosynthesis

Published Open-Access

yes

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