Language

English

Publication Date

10-1-2023

Journal

Current Opinion in Structural Biology

DOI

10.1016/j.sbi.2023.102647

PMID

37399693

PMCID

PMC10527939

PubMedCentral® Posted Date

10-1-2024

PubMedCentral® Full Text Version

Author MSS

Abstract

Polymyxins, a last resort antibiotic, target the outer membrane of pathogens and are used to address the increasing prevalence of multidrug-resistant Gram-negative bacteria. The plasmid-encoded enzyme MCR-1 confers polymyxin resistance to bacteria by modifying the outer membrane. Transferable resistance to polymyxins is a major concern; therefore, MCR-1 is an important drug target. In this review, we discuss recent structural and mechanistic aspects of MCR-1 function, its variants and homologs, and how they are relevant to polymyxin resistance. Specifically, we discuss work on polymyxin-mediated disruption of the outer and inner membranes, computational studies on the catalytic mechanism of MCR-1, mutagenesis and structural analysis concerning residues important for substrate binding in MCR-1, and finally, advancements in inhibitors targeting MCR-1.

Keywords

Polymyxins, Colistin, Escherichia coli Proteins, Drug Resistance, Bacterial, Anti-Bacterial Agents, Biology

Published Open-Access

yes

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