Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis

Authors

Mark R Tanner
Redwan Huq
William K A Sikkema
Lizanne G Nilewski
Nejla Yosef
Cody Schmitt
Carlos P Flores-Suarez
Arielle Raugh
Teresina Laragione
Pércio S Gulko
James M Tour
Christine Beeton

Language

English

Publication Date

10-16-2020

Journal

Antioxidants

DOI

10.3390/antiox9101005

PMID

33081234

PMCID

PMC7602875

PubMedCentral® Posted Date

10-16-2020

PubMedCentral® Full Text Version

Post-print

Abstract

Reactive oxygen species have been involved in the pathogenesis of rheumatoid arthritis (RA). Our goal was to determine the effects of selectively scavenging superoxide (O2•−) and hydroxyl radicals with antioxidant nanoparticles, called poly(ethylene glycol)-functionalized hydrophilic carbon clusters (PEG-HCCs), on the pathogenic functions of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA) and on the progression of an animal model of RA. We used human FLS from patients with RA to determine PEG-HCC internalization and effects on FLS cytotoxicity, invasiveness, proliferation, and production of proteases. We used the pristane-induced arthritis (PIA) rat model of RA to assess the benefits of PEG-HCCs on reducing disease severity. PEG-HCCs were internalized by RA-FLS, reduced their intracellular O2•−, and reduced multiple measures of their pathogenicity in vitro, including proliferation and invasion. In PIA, PEG-HCCs caused a 65% reduction in disease severity, as measured by a standardized scoring system of paw inflammation and caused a significant reduction in bone and tissue damage, and circulating rheumatoid factor. PEG-HCCs did not induce lymphopenia during PIA. Our study demonstrated a role for O2•− and hydroxyl radicals in the pathogenesis of a rat model of RA and showed efficacy of PEG-HCCs in treating a rat model of RA.

Keywords

synovial fibroblast, oxidative stress, nanomaterials

Published Open-Access

yes

Recommended Citation

Tanner, Mark R; Huq, Redwan; Sikkema, William K A; et al., "Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis" (2020). Faculty and Staff Publications. 658.
https://digitalcommons.library.tmc.edu/baylor_docs/658