Language
English
Publication Date
12-1-2025
Journal
Journal of Leukocyte Biology
DOI
10.1093/jleuko/qiaf160
PMID
41206685
PMCID
PMC12683237
PubMedCentral® Posted Date
11-7-2025
PubMedCentral® Full Text Version
Post-print
Abstract
Regulatory T cells (Tregs) play a crucial role in the immune system, and their dysfunction can lead to the development of autoimmune conditions. In cancer, tumors frequently hijack the immunosuppressive function of Tregs to evade immune responses. Due to their central role in key pathological processes, Tregs have gained increasing attention as promising targets for various clinical applications. However, their relative scarcity (∼5% to 10% of CD4+ T cells) and instability presents a technical challenge for research and therapeutic development. In congenic animal models used to investigate autologous cell transfer-based therapies, this challenge is even greater, as Treg donor animals may only be able to provide cells to a small number of recipient mice. Here, we present an optimized protocol for ex vivo editing and expansion of mouse Tregs. Because a recent study demonstrated the anticancer potential of SRC-3 KO mouse Tregs, we use them here as a case study.
Keywords
Animals, T-Lymphocytes, Regulatory, Mice, Mice, Knockout, Gene Editing, Mice, Inbred C57BL, cell therapy, CRISPR-Cas9, gene editing, SRC-3, Tregs
Published Open-Access
yes
Recommended Citation
Gilad, Yosef; Dean, Adam M; Xia, Yan; et al., "Method for Generation and Ex Vivo Expansion of Genetically Edited Mouse Tregs" (2025). Faculty, Staff and Students Publications. 7061.
https://digitalcommons.library.tmc.edu/baylor_docs/7061