Publication Date

12-1-2026

Journal

Channels

DOI

10.1080/19336950.2026.2687246

PMID

42328935

PMCID

PMC13290088

PubMedCentral® Posted Date

6-22-2026

PubMedCentral® Full Text Version

Post-print

Abstract

Hyperexcitability is a biomarker of early-stage Alzheimer’s Disease (AD) and hastens cognitive decline later in its course. Mechanistic target of rapamycin (mTOR) signaling contributes to the slope of this trajectory, as evidenced by early increased brain expression and the rescue of hyperexcitability by genetic deletion of mTOR complex 2 (mTORC2); however, a molecular mechanism directly linking mTOR signaling to membrane hyperexcitability in early-stage AD remains elusive. Here, we show that hyperactive mTOR signaling stimulates the voltage-gated Na+ channel 1.2 (Nav1.2), a previously identified downstream phosphorylation target of mTORC2 and a key regulator of membrane electrogenesis. Augmented Nav1.2 channel function induced by hyperactive mTOR signaling requires the action of mTORC2 and is selective among major brain Nav channel isoforms. In a murine AD model, neocortical pyramidal neurons display augmented Nav1.2 channel function and hyperexcitability through a mechanism that requires mTORC2 activity. These results highlight the mTORC2-Nav1.2 interaction as a therapeutic target for attenuating pathogenic hyperexcitability in early-stage AD.

Keywords

Animals, Alzheimer Disease, Mice, Signal Transduction, Disease Models, Animal, Mechanistic Target of Rapamycin Complex 2, NAV1.2 Voltage-Gated Sodium Channel, Humans, Voltage-gated Na+ channel, mechanistic target of rapamycin, Alzheimer’s disease, hyperexcitability, patch-clamp electrophysiology

Published Open-Access

yes

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