Language

English

Publication Date

4-1-2024

Journal

Journal of Clinical Immunology

DOI

10.1016/j.clim.2024.109942

PMID

38367737

PMCID

PMC11018339

PubMedCentral® Posted Date

4-1-2026

PubMedCentral® Full Text Version

Author MSS

Abstract

Severe combined immunodeficiency (SCID) is characterized by a severe deficiency in T cell numbers. We analyzed data collected (n = 307) for PHA-based T cell proliferation from the PIDTC SCID protocol 6901, using either a radioactive or flow cytometry method. In comparing the two groups, a smaller number of the patients tested by flow cytometry had < 10% of the lower limit of normal proliferation as compared to the radioactive method (p = 0.02). Further, in patients with CD3+ T cell counts between 51 and 300 cells/μL, there was a higher proliferative response with the PHA flow assay compared to the 3H-T assay (p < 0.0001), suggesting that the method of analysis influences the resolution and interpretation of PHA results. Importantly, we observed many SCID patients with profound T cell lymphopenia having normal T cell proliferation when assessed by flow cytometry. We recommend this test be considered only as supportive in the diagnosis of typical SCID.

Keywords

Infant, Newborn, Humans, Severe Combined Immunodeficiency, Lymphopenia, Neonatal Screening, T-Lymphocytes, Cell Proliferation, PHA, Mitogen, SCID, Severe combined immunodeficiency, PIDTC, T-cell proliferation, T-cell lymphopenia

Published Open-Access

yes

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