Language
English
Publication Date
1-4-2023
Journal
International Journal of Molecular Sciences
DOI
10.3390/ijms24020931
PMID
36674448
PMCID
PMC9865780
PubMedCentral® Posted Date
1-4-2023
PubMedCentral® Full Text Version
Post-print
Abstract
High-fat/sucrose diet feeding in mice causes loss of corneal nerve function and impairs corneal wound healing. While changing to a diet with a low fat/sugar composition and enrichments in complex carbohydrates mitigates the reduction in nerve function, it remains to be determined if it has an effect on corneal wound healing. In this study, 6-week-old C57BL/6 male mice were fed either a normal diet or a high-fat/sucrose diet for 20 weeks. A third group (diet reversal) was placed on a high-fat/sucrose diet for 10 weeks followed by a normal diet for an additional 10 weeks. A central corneal epithelial abrasion wound was created, and wound closure was monitored. Neutrophil and platelet recruitment was assessed by immunofluorescence microscopy. Mice fed the high-fat/sucrose diet-only had greater adiposity (p < 0.005) than normal diet-only fed mice; diet reversal markedly reduced adiposity. Following corneal abrasion, wound closure was delayed by ~6 h (p ≤ 0.01) and, at 30 h post-wounding, fewer neutrophils reached the wound center and fewer extravascular platelets were present at the limbus (p < 0.05). Diet restored normal wound closure and neutrophil and platelet influx in the injured cornea. These data suggest compositional changes to the diet may be an effective diet-based therapeutic strategy for maintaining or restoring corneal health.
Keywords
Male, Animals, Mice, Sucrose, Mice, Inbred C57BL, Cornea, Corneal Injuries, Obesity, Diet, High-Fat, cornea, macronutrient, high-fat/sucrose diet, obesity, neutrophils, platelets
Published Open-Access
yes
Recommended Citation
Akowuah, Prince K; Lema, Carolina; Rumbaut, Rolando E; et al., "A Low-Fat/Sucrose Diet Rich in Complex Carbohydrates Reverses High-Fat/Sucrose Diet-Induced Corneal Dysregulation" (2023). Faculty and Staff Publications. 788.
https://digitalcommons.library.tmc.edu/baylor_docs/788
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Eye Diseases Commons, Hematology Commons, Neurology Commons, Ophthalmology Commons