Publication Date
1-4-2023
Journal
International Journal of Molecular Sciences
DOI
10.3390/ijms24020931
PMID
36674448
PMCID
PMC9865780
PubMedCentral® Posted Date
1-4-2023
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Male, Animals, Mice, Sucrose, Mice, Inbred C57BL, Cornea, Corneal Injuries, Obesity, Diet, High-Fat, cornea, macronutrient, high-fat/sucrose diet, obesity, neutrophils, platelets
Abstract
High-fat/sucrose diet feeding in mice causes loss of corneal nerve function and impairs corneal wound healing. While changing to a diet with a low fat/sugar composition and enrichments in complex carbohydrates mitigates the reduction in nerve function, it remains to be determined if it has an effect on corneal wound healing. In this study, 6-week-old C57BL/6 male mice were fed either a normal diet or a high-fat/sucrose diet for 20 weeks. A third group (diet reversal) was placed on a high-fat/sucrose diet for 10 weeks followed by a normal diet for an additional 10 weeks. A central corneal epithelial abrasion wound was created, and wound closure was monitored. Neutrophil and platelet recruitment was assessed by immunofluorescence microscopy. Mice fed the high-fat/sucrose diet-only had greater adiposity (p < 0.005) than normal diet-only fed mice; diet reversal markedly reduced adiposity. Following corneal abrasion, wound closure was delayed by ~6 h (p ≤ 0.01) and, at 30 h post-wounding, fewer neutrophils reached the wound center and fewer extravascular platelets were present at the limbus (p < 0.05). Diet restored normal wound closure and neutrophil and platelet influx in the injured cornea. These data suggest compositional changes to the diet may be an effective diet-based therapeutic strategy for maintaining or restoring corneal health.
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Eye Diseases Commons, Hematology Commons, Neurology Commons, Ophthalmology Commons
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