Language
English
Publication Date
1-4-2024
Journal
bioRxiv
DOI
10.1101/2023.10.07.561364
PMID
37873455
PMCID
PMC10592978
PubMedCentral® Posted Date
1-4-2024
PubMedCentral® Full Text Version
Post-print
Abstract
Numerous components of the transcription machinery, including RNA polymerase II (Pol II), accumulate in regions of high local concentration known as clusters, which are thought to facilitate transcription. Using the histone locus of Drosophila nurse cells as a model, we find that Pol II forms long-lived, transcriptionally poised clusters distinct from liquid droplets, which contain unbound and paused Pol II. Depletion of the Integrator complex endonuclease module, but not its phosphatase module or Pol II pausing factors disperses these Pol II clusters. Consequently, histone transcription fails to reach peak levels during S-phase and aberrantly continues throughout the cell cycle. We propose that Pol II clustering is a regulatory step occurring near promoters that limits rapid gene activation to defined times.
Published Open-Access
yes
Recommended Citation
Wilusz, Jeremy E; Gilmour, David S; Lehmann, Ruth; et al., "Integrator-Mediated Clustering of Poised RNA Polymerase II Synchronizes Histone Transcription" (2024). Faculty, Staff and Students Publications. 8320.
https://digitalcommons.library.tmc.edu/baylor_docs/8320