Protocol for Quantification of Aav-Crispr-Mediated Hepatic Gene Editing in Mice

Authors

• Precious Juvie Calderon
• Rochelle W Lai
• Angela Cheng
• Manasvi Paudel
• Gabriella E Rubert
• Anthony Suberski
• Owen K Traina
• Sunny Y Cai
• Kalyani Srikanth
• William R Lagor
• Elizabeth J Tarling
• Thomas Q de Aguiar Vallim

Language

English

Publication Date

9-29-2026

Journal

STAR Protocols

DOI

10.1016/j.xpro.2026.104849

PMID

42809437

PMCID

PMC13654526

PubMedCentral® Posted Date

9-29-2026

PubMedCentral® Full Text Version

Post-print

Abstract

Adeno associated viral (AAV) delivery of CRISPR-Cas9 enables efficient, targeted hepatic gene disruption composed of both small insertions and deletions (INDELs) and larger DNA structural variants (DNA-SVs), which are difficult to quantify. Here, we present a protocol for quantifying AAV-CRISPR-mediated gene editing in mice. We describe guide RNA (gRNA) design, plasmid cloning, delivery of AAV-CRISPR to the liver, and execution of liver DNA-editing analysis. This protocol enables quantification of INDELs and DNA-SVs, allowing for validation of gene disruption.

For complete details on the use and execution of this protocol, please refer to Chan et al.1

Keywords

Metabolism, Molecular Biology, CRISPR

Published Open-Access

yes

Recommended Citation

Calderon, Precious Juvie; Lai, Rochelle W; Cheng, Angela; et al., "Protocol for Quantification of Aav-Crispr-Mediated Hepatic Gene Editing in Mice" (2026). Faculty, Staff and Students Publications. 8583.
https://digitalcommons.library.tmc.edu/baylor_docs/8583