Duncan NRI Faculty and Staff Publications

Language

English

Publication Date

3-4-2022

Journal

Science

DOI

10.1126/science.abk2432

PMID

35239393

PMCID

PMC8944923

PubMedCentral® Posted Date

3-24-2022

PubMedCentral® Full Text Version

Author MSS

Abstract

For more than 100 years, the fruit fly Drosophila melanogaster has been one of the most studied model organisms. Here, we present a single-cell atlas of the adult fly, Tabula Drosophilae, that includes 580,000 nuclei from 15 individually dissected sexed tissues as well as the entire head and body, annotated to >250 distinct cell types. We provide an in-depth analysis of cell type-related gene signatures and transcription factor markers, as well as sexual dimorphism, across the whole animal. Analysis of common cell types between tissues, such as blood and muscle cells, reveals rare cell types and tissue-specific subtypes. This atlas provides a valuable resource for the Drosophila community and serves as a reference to study genetic perturbations and disease models at single-cell resolution.

Keywords

Animals, Cell Nucleus, Databases, Genetic, Drosophila Proteins, Drosophila melanogaster, Female, Gene Expression Regulation, Gene Regulatory Networks, Genes, Insect, Male, RNA-Seq, Sex Characteristics, Single-Cell Analysis, Transcription Factors, Transcriptome

Published Open-Access

yes

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