Not Feeling the Heat? Effects of Dietary Protein on Satiation and Satiety in Mice Are Not Due to Its Impact on Body Temperature

Authors

• Jazmin Osorio M
• Sharon E Mitchell
• Catherine Hambly
• David B Allison
• John R Speakman

Language

English

Publication Date

9-1-2024

Journal

Appetite

DOI

10.1016/j.appet.2024.107421

PMID

38759755

Abstract

Dietary protein modulates food intake (FI) via unclear mechanism(s). One possibility is that higher protein leads to greater post-ingestive heat production (Specific dynamic action: SDA) leading to earlier meal termination (increased satiation), and inhibition of further intake (increased satiety). The influence of dietary protein on feeding behaviour in C57BL/6J mice was tested using an automated FI monitoring system (BioDAQ), simultaneous to body temperature (Tb). Total FI, inter meal intervals (IMI, satiety) and meal size (MS, satiation) were related to changes in Tb after consuming low (5%, LP), moderate (15%, MP) and high (30%, HP) protein diets. Diets were tested over three conditions: 1) room temperature (RT, 21 ± 1 °C), 2) room temperature and running wheels (RTRW) and 3) low temperature (10 °C) and running wheels (LTRW). The differences between diets and conditions were also compared using mixed models. Mice housed at RT fed HP diet, reduced total FI compared with LP and MP due to earlier meal termination (satiation effect). FI was lowered in RTRW conditions with no differences between diets. FI significantly increased under LTRW conditions for all diets, with protein content leading to earlier meal termination (satiation) but not the intervals between feeding bouts (satiety). Tb fell immediately after feeding in all conditions. Despite a reduction in total FI in mice fed HP, mediated via increased satiation, this effect was not linked to increased Tb during meals. We conclude effects of dietary protein on intake are not mediated via SDA and Tb.

Keywords

Animals, Mice, Inbred C57BL, Satiation, Dietary Proteins, Male, Mice, Body Temperature, Feeding Behavior, Eating, Satiety Response, Body temperature, Dietary protein, Hunger, Satiation, Specific dynamic action

Published Open-Access

yes

Recommended Citation

Osorio M, Jazmin; Mitchell, Sharon E; Hambly, Catherine; et al., "Not Feeling the Heat? Effects of Dietary Protein on Satiation and Satiety in Mice Are Not Due to Its Impact on Body Temperature" (2024). Children’s Nutrition Research Center Staff Publications. 320.
https://digitalcommons.library.tmc.edu/staff_pub/320