Faculty, Staff and Student Publications
Language
English
Publication Date
8-1-2025
Journal
Clinical and Experimental Dental Research
DOI
10.1002/cre2.70189
PMID
40741849
PMCID
PMC12311837
PubMedCentral® Posted Date
7-31-2025
PubMedCentral® Full Text Version
Post-print
Abstract
Objectives: This study aimed to evaluate the antimicrobial efficacy of three commercially available denture cleaning tablets Efferdent, Polident, and Val-clean against Staphylococcus aureus biofilms formed on two denture base materials: milled polymethyl methacrylate (PMMA) and 3D printed denture resin.
Material and methods: Eighty samples were fabricated, with 40 specimens per denture base material: CAD/CAM milled PMMA (Lucitone Digital Fit LT; Dentsply Sirona, Charlotte, NC, USA) and 3D printed resin (Denture Base Resin; Formlabs Inc., Somerville, MA, USA). Each group was divided into four subgroups (n = 10), Efferdent, Polident, Val-clean, and deionized water (control). Surface roughness was measured using a digital profilometer (Digiprofilo I; Digiwork Instruments, Concord, ON, Canada). Samples were exposed to S. aureus in tryptic soy broth (TSB) and subsequently treated with the cleaning solutions. Colony-forming units (CFUs) were quantified using serial dilution plating. To distinguish bactericidal from bacteriostatic effects, normalized S. aureus cultures were incubated with the cleaning agents for 16 h, followed by optical density measurements, LIVE/DEAD staining (Invitrogen, Carlsbad, California), and TSB agar plating. Statistical analysis was performed using one-way ANOVA and Dunnett's multiple comparisons test, with significance set at p < 0.05.
Results: A significant difference in surface roughness was observed between the 3D printed and milled samples before treatment (p < 0.0001). Significantly fewer CFUs were observed in all cleaning solution groups compared to the control (p < 0.05). All denture cleaning solutions demonstrated bactericidal activity against S. aureus, as indicated by the absence of CFU growth on agar plates and the lack of viable cells in LIVE/DEAD staining posttreatment. Both denture materials demonstrated comparable microbial adhesion and response to the cleaning agents.
Conclusions: Efferdent, Polident, and Val-clean denture tablets are effective bactericidal agents against S. aureus in vitro, regardless of the denture base material used. These findings support their use in daily denture hygiene routines, particularly in reducing bacterial colonization on removable prostheses.
Keywords
Staphylococcus aureus, Denture Bases, Denture Cleansers, Biofilms, Polymethyl Methacrylate, Surface Properties, Printing, Three-Dimensional, Tablets, Dental Materials, Humans, Materials Testing, Acrylic Resins, Borates, Sulfates, dentures, Efferdent, Polident, Staphylococcus aureus, Val‐clean
Published Open-Access
yes
Recommended Citation
Vishaj, Sindi; Villhauer, Alissa; Morris, Mandi; et al., "Evaluating the Antimicrobial Properties of Different Dental Cleaning Tablets Against S aureus Grown on Two Denture Base Materials" (2025). Faculty, Staff and Student Publications. 154.
https://digitalcommons.library.tmc.edu/uthdb_docs/154