Functionalized Scaffold and Barrier Membrane with Anti-BMP-2 Monoclonal Antibodies for Alveolar Ridge Preservation in a Canine Model

Authors

Seiko Min
Taewan Kim
Oksu Kim
Carames Goncalo
Tadahiko Utsunomiya
Takashi Matsumoto
Kayo Kuyama
Nikola Angelov

Publication Date

1-1-2020

Journal

Biomedical Research International

Abstract

INTRODUCTION: The aim of this study was to investigate the ability of anti-bone morphogenetic protein 2 monoclonal antibody (anti-BMP-2 mAb) to functionalize scaffolds to mediate bone regeneration in a canine model.

MATERIALS AND METHODS: The mandibular right premolar 4 (PM4) was extracted in eight beagle dogs and grafted with anti-BMP-2 mAb+anorganic bovine bone mineral with 10% collagen (ABBM-C) and porcine bilayer native collagen membrane (CM). The ABBM-C and CM were functionalized with either anti-BMP-2 mAb (test group) or an isotype matched control mAb (control group). Animals were euthanized at 12 weeks for radiographic, histologic, and histomorphometric analyses. Outcomes were compared between groups.

RESULTS: 3D imaging using cone beam computed tomography (CBCT) revealed that sites treated with ABBM-C and CM functionalized with anti-BMP-2 mAb exhibited significantly more remaining bone width near the alveolar crest, as well as buccal bone height, compared with control groups. Histologic and histomorphometric analyses demonstrated that in anti-BMP-2 mAb-treated sites, total tissue volume was significantly higher in the coronal part of the alveolar bone crest compared with control sites. In anti-BMP-2 mAb-treated sites, bone formation was observed under the barrier membrane.

CONCLUSION: Functionalization of the ABBM-C scaffold and CM appeared to have led to bone formation within healing alveolar bone sockets.

Keywords

Alveolar Process, Anatomic Landmarks, Animals, Antibodies, Monoclonal, Bicuspid, Bone Morphogenetic Protein 2, Cone-Beam Computed Tomography, Disease Models, Animal, Dogs, Mandible, Membranes, Organ Size, Tissue Scaffolds

Recommended Citation

Min, Seiko; Kim, Taewan; Kim, Oksu; Goncalo, Carames; Utsunomiya, Tadahiko; Matsumoto, Takashi; Kuyama, Kayo; and Angelov, Nikola, "Functionalized Scaffold and Barrier Membrane with Anti-BMP-2 Monoclonal Antibodies for Alveolar Ridge Preservation in a Canine Model" (2020). Faculty, Staff and Student Publications. 33.
https://digitalcommons.library.tmc.edu/uthdb_docs/33