Loss of Sc5d Results in Micrognathia Due to a Failure in Osteoblast Differentiation

Authors

Language

English

Publication Date

11-1-2024

Journal

Journal of Advanced Research

DOI

10.1016/j.jare.2023.12.008

PMID

38086515

PMCID

PMC11519736

PubMedCentral® Posted Date

12-10-2023

PubMedCentral® Full Text Version

Post-print

Abstract

INTRODUCTION: Mutations in genes related to cholesterol metabolism, or maternal diet and health status, affect craniofacial bone formation. However, the precise role of intracellular cholesterol metabolism in craniofacial bone development remains unclear.

OBJECTIVE: The aim of this study is to determine how cholesterol metabolism aberrations affect craniofacial bone development.

METHODS: Mice with a deficiency in Sc5d, which encodes an enzyme involved in cholesterol synthesis, were analyzed with histology, micro computed tomography (microCT), and cellular and molecular biological methods.

RESULTS: Sc5d null mice exhibited mandible hypoplasia resulting from defects in osteoblast differentiation. The activation of the hedgehog and WNT/β-catenin signaling pathways, which induce expression of osteogenic genes Col1a1 and Spp1, was compromised in the mandible of Sc5d null mice due to a failure in the formation of the primary cilium, a cell surface structure that senses extracellular cues. Treatments with an inducer of hedgehog or WNT/β-catenin signaling or with simvastatin, a drug that restores abnormal cholesterol production, partially rescued the defects in osteoblast differentiation seen in Sc5d mutant cells.

CONCLUSION: Our results indicate that loss of Sc5d results in mandibular hypoplasia through defective primary cilia-mediated hedgehog and WNT/β-catenin signaling pathways.

Keywords

Animals, Mice, Cell Differentiation, Cholesterol, Cilia, Hedgehog Proteins, Mandible, Mice, Knockout, Micrognathism, Osteoblasts, Osteogenesis, Simvastatin, Wnt Signaling Pathway, Oxidoreductases Acting on CH-CH Group Donors, Craniofacial bone formation, Cholesterol metabolism, Sc5d, Mandible hypoplasia, Primary cilia

Published Open-Access

yes

Recommended Citation

, "Loss of Sc5d Results in Micrognathia Due to a Failure in Osteoblast Differentiation" (2024). Faculty, Staff and Student Publications. 69.
https://digitalcommons.library.tmc.edu/uthdb_docs/69