Student and Faculty Publications
Publication Date
7-14-2023
Journal
The Journal of Infectious Disease
Abstract
We analyzed findings in a same-gender couple discordant in their human immunodeficiency virus (HIV) status. The HIV+ partner was homozygous for CCR5 while his receptive HIV- partner was a CCR5Δ32 heterozygote with a C20S missense mutation in his CCR5 allele. The cells from the HIV- partner showed significant resistance to R5 fusion/infection and had no chemotactic response to CCL4 (macrophage inflammatory protein 1β). We demonstrated abundant CCR5-specific RNA in the HIV- partner's cells but no detectable CCR5 protein. CCR5 promoter region cloned from each partner's DNA indicated no significant impact on RNA transcription. The compound effect of CCR5Δ32 and C20S mutation impaired CCR5 coreceptor function and conferred resistance to HIV-1.
Keywords
HIV Infections, Humans, Disease Resistance, HIV-1, Receptors, CCR5, Male, Mutation, Missense, CCR5, CCR5Δ32, HIV/AIDS, heterozygous, homozygous
Included in
Bioinformatics Commons, Biomedical Informatics Commons, Infectious Disease Commons, Medical Sciences Commons, Oncology Commons
Comments
Supplementary Materials
PMID: 36912158