Enzymatic Characterization of mRNA Cap Adenosine-N6 Methyltransferase PCIF1 Activity on Uncapped RNAs

Authors

Dan Yu
Nan Dai
Eric J Wolf
Ivan R Corrêa
Jujun Zhou
Tao Wu
Robert M Blumenthal
Xing Zhang
Xiaodong Cheng

Publication Date

4-1-2022

Journal

Journal of Biological Chemistry

Abstract

The phosphorylated RNA polymerase II CTD interacting factor 1 (PCIF1) is a methyltransferase that adds a methyl group to the N6-position of 2′O-methyladenosine (Am), generating N6, 2′O-dimethyladenosine (m6Am) when Am is the cap-proximal nucleotide. In addition, PCIF1 has ancillary methylation activities on internal adenosines (both A and Am), although with much lower catalytic efficiency relative to that of its preferred cap substrate. The PCIF1 preference for 2′O-methylated Am over unmodified A nucleosides is due mainly to increased binding affinity for Am. Importantly, it was recently reported that PCIF1 can methylate viral RNA. Although some viral RNA can be translated in the absence of a cap, it is unclear what roles PCIF1 modifications may play in the functionality of viral RNAs. Here we show, using in vitro assays of binding and methyltransfer, that PCIF1 binds an uncapped 5′-Am oligonucleotide with approximately the same affinity as that of a cap analog (KM = 0.4 versus 0.3 μM). In addition, PCIF1 methylates the uncapped 5′-Am with activity decreased by only fivefold to sixfold compared with its preferred capped substrate. We finally discuss the relationship between PCIF1-catalyzed RNA methylation, shown here to have broader substrate specificity than previously appreciated, and that of the RNA demethylase fat mass and obesity-associated protein (FTO), which demonstrates PCIF1-opposing activities on capped RNAs.

Keywords

Adaptor Proteins, Signal Transducing, Adenosine, Humans, Methyltransferases, Nuclear Proteins, Protein Binding, RNA Caps, RNA, Viral, RNA adenine methylation, N6, 2′O-dimethyladenosine, uncapped RNAs, viral mRNA

DOI

doi: 10.1016/j.jbc.2022.101751

PMID

35189146

PMCID

PMC8931429

PubMedCentral® Posted Date

2-19-2022

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes