Excess adenosine in murine penile erectile tissues contributes to priapism via A2B adenosine receptor signaling

Authors

Tiejuan Mi
Shahrzad Abbasi
Hong Zhang
Karen Uray
Janci L Chunn
Ling Wei Xia
Jose G Molina
Norman W Weisbrodt
Rodney E Kellems
Michael R Blackburn
Yang Xia

Publication Date

4-1-2008

Journal

Journal of Clinical Investigation

Abstract

Priapism, abnormally prolonged penile erection in the absence of sexual excitation, is associated with ischemia-mediated erectile tissue damage and subsequent erectile dysfunction. It is common among males with sickle cell disease (SCD), and SCD transgenic mice are an accepted model of the disorder. Current strategies to manage priapism suffer from a poor fundamental understanding of the molecular mechanisms underlying the disorder. Here we report that mice lacking adenosine deaminase (ADA), an enzyme necessary for the breakdown of adenosine, displayed unexpected priapic activity. ADA enzyme therapy successfully corrected the priapic activity both in vivo and in vitro, suggesting that it was dependent on elevated adenosine levels. Further genetic and pharmacologic evidence demonstrated that A2B adenosine receptor-mediated (A2BR-mediated) cAMP and cGMP induction was required for elevated adenosine-induced prolonged penile erection. Finally, priapic activity in SCD transgenic mice was also caused by elevated adenosine levels and A2BR activation. Thus, we have shown that excessive adenosine accumulation in the penis contributes to priapism through increased A2BR signaling in both Ada -/- and SCD transgenic mice. These findings provide insight regarding the molecular basis of priapism and suggest that strategies to either reduce adenosine or block A2BR activation may prove beneficial in the treatment of this disorder.

Keywords

Adenosine, Adenosine Deaminase, Animals, Cyclic AMP, Cyclic GMP, Fibrosis, Male, Mice, Mice, Knockout, Myocytes, Smooth Muscle, Penile Erection, Penis, Receptor, Adenosine A2B, Signal Transduction, Time Factors