Recombinant Protein Micelles to Block Transduction by SARS-CoV-2 Pseudovirus

Authors

Rajarshi Chattaraj
Christina Y Kim
Daeyeon Lee
Daniel A Hammer

Language

English

Publication Date

10-25-2022

Journal

ACS Nano

DOI

10.1021/acsnano.2c09015

PMID

36191145

PMCID

PMC9578646

PubMedCentral® Posted Date

October 2023

PubMedCentral® Full Text Version

Author MSS

Abstract

The continuing emergence of variants of the SARS-CoV-2 virus requires the development of modular molecular therapies. Here, we engineered a recombinant amphiphilic protein, oleosin, to spontaneously self-assemble into multivalent micellar nanostructures which can block the Spike S1 protein of SARS-CoV-2 pseudoviruses (PVs). Short recombinant proteins like oleosin can be formulated more easily than antibodies and can be functionalized with precision through genetic engineering. We cloned S1-binding mini-protein genes called LCB

Keywords

Humans, Micelles, SARS-CoV-2, Escherichia coli, COVID-19, Recombinant Proteins, Peptides

Published Open-Access

yes

Recommended Citation

Chattaraj, Rajarshi; Kim, Christina Y; Lee, Daeyeon; et al., "Recombinant Protein Micelles to Block Transduction by SARS-CoV-2 Pseudovirus" (2022). Faculty, Staff and Student Publications. 1463.
https://digitalcommons.library.tmc.edu/uthmed_docs/1463