Hepatic and renal cytochrome p450 gene regulation during citrobacter rodentium infection in wild-type and toll-like receptor 4 mutant mice

Authors

Terrilyn A Richardson
Melanie Sherman
Leposava Antonovic
Sean S Kardar
Henry W Strobel
Daniel Kalman
Edward T Morgan

Publication Date

3-1-2006

Journal

Drug Metabolism and Disposition

Abstract

Citrobacter rodentium is the rodent equivalent of human enteropathogenic Escherichia coli infection. This study investigated regulation of hepatic and renal cytochrome P450 (P450) mRNAs, hepatic P450 proteins, cytokines, and acute phase proteins during C. rodentium infection. Female C3H/HeOuJ (HeOu) and C3H/HeJ (HeJ) mice [which lack functional toll-like receptor 4 (TLR4)] were infected with C. rodentium by oral gavage and sacrificed 6 days later. Hepatic CYP4A10 and 4A14 mRNAs were decreased in HeOu mice (

Keywords

Animals, Blotting, Western, Citrobacter rodentium, Cytochrome P-450 Enzyme System, Enterobacteriaceae Infections, Female, Gene Expression Regulation, Enzymologic, Kidney, Liver, Mice, Mice, Mutant Strains, Mutation, Reverse Transcriptase Polymerase Chain Reaction, Toll-Like Receptor 4