Faculty, Staff and Student Publications
Language
English
Publication Date
10-1-2023
Journal
Translational Stroke Research
DOI
10.1007/s12975-022-01070-z
PMID
35906327
PMCID
PMC10490444
PubMedCentral® Posted Date
October 2023
PubMedCentral® Full Text Version
Author MSS
Abstract
Post-menopausal women become vulnerable to stroke and have poorer outcomes and higher mortality than age-matched men, and previous studies suggested that sex chromosomes play a vital role in mediating stroke sensitivity in the aged. It is unknown if this is due to effects of the X or Y chromosome. The present study used the XY* mouse model (with four genotypes: XX and XO gonadal females and XY and XXY gonadal males) to compare the effect of the X vs. Y chromosome compliment in stroke. Aged (18-20 months) and gonadectomized young (8-12 weeks) mice were subjected to a 60-min middle cerebral artery occlusion. Infarct volume and behavioral deficits were quantified 3 days after stroke. Microglial activation and infiltration of peripheral leukocytes in the aged ischemic brain were assessed by flow cytometry. Plasma inflammatory cytokine levels by ELISA, and brain expression of two X chromosome-linked genes, KDM6A and KDM5C by immunochemistry, were also examined. Both aged and young XX and XXY mice had worse stroke outcomes compared to XO and XY mice, respectively; however, the difference between XX vs. XXY and XO vs. XY aged mice was minimal. Mice with two copies of the X chromosome showed more robust microglial activation, higher brain-infiltrating leukocytes, elevated plasma cytokine levels, and enhanced co-localization of KDM6A and KDM5C with Iba1
Keywords
Microglia, Neuroinflammation, Sex chromosome, Stroke, XY* mice
Published Open-Access
yes
Recommended Citation
Qi, Shaohua; Ngwa, Conelius; Al Mamun, Abdullah; et al., "X, But Not Y, Chromosomal Complement Contributes To Stroke Sensitivity In Aged Animals" (2023). Faculty, Staff and Student Publications. 1490.
https://digitalcommons.library.tmc.edu/uthmed_docs/1490