PMCA for Ultrasensitive Detection of Prions and to Study Disease Biology

Authors

Fei Wang
Sandra Pritzkow
Claudio Soto

Language

English

Publication Date

4-1-2023

Journal

Cell Tissue Research

DOI

10.1007/s00441-022-03727-5

PMID

36567368

PMCID

PMC9790818

PubMedCentral® Posted Date

December 2022

PubMedCentral® Full Text Version

Post-print

Abstract

The emergence of a novel class of infectious agent composed exclusively of a misfolded protein (termed prions) has been a challenge in modern biomedicine. Despite similarities on the behavior of prions with respect to conventional pathogens, the many uncertainties regarding the biology and virulence of prions make them a worrisome paradigm. Since prions do not contain nucleic acids and rely on a very different way of replication and spreading, it was necessary to invent a novel technology to study them. In this article, we provide an overview of such a technology, termed protein misfolding cyclic amplification (PMCA), and summarize its many applications to detect prions and understand prion biology.

Keywords

Prions, Protein misfolding cyclic amplification, Biomedicine

Published Open-Access

yes

Recommended Citation

Fei Wang, Sandra Pritzkow, and Claudio Soto, "PMCA for Ultrasensitive Detection of Prions and to Study Disease Biology" (2023). Faculty, Staff and Student Publications. 1542.
https://digitalcommons.library.tmc.edu/uthmed_docs/1542