The CTSA University of Texas Health Science Center (UTHSC) Northeast—Tyler and Rio Grande Valley Success Story: How Rural, Underserved Academic Communities Rapidly Built a Robust Engine for Collaborative COVID-19 Clinical Research

Authors

Daisuke Shiomi
William Margolin

Language

English

Publication Date

1-1-2007

Journal

Journal of Bacteriology

DOI

10.1128/JB.00666-06

PMID

17085577

PMCID

PMC1797224

PubMedCentral® Posted Date

November 2006

PubMedCentral® Full Text Version

Post-print

Abstract

In Escherichia coli, the Min system, consisting of three proteins, MinC, MinD, and MinE, negatively regulates FtsZ assembly at the cell poles, helping to ensure that the Z ring will assemble only at midcell. Of the three Min proteins, MinC is sufficient to inhibit Z-ring assembly. By binding to MinD, which is mostly localized at the membrane near the cell poles, MinC is sequestered away from the cell midpoint, increasing the probability of Z-ring assembly there. Previously, it has been shown that the two halves of MinC have two distinct functions. The N-terminal half is sufficient for inhibition of FtsZ assembly, whereas the C-terminal half of the protein is required for binding to MinD as well as to a component of the division septum. In this study, we discovered that overproduction of the C-terminal half of MinC (MinC(122-231)) could also inhibit cell division and that this inhibition was at the level of Z-ring disassembly and dependent on MinD. We also found that fusing green fluorescent protein to either the N-terminal end of MinC(122-231), the C terminus of full-length MinC, or the C terminus of MinC(122-231) perturbed MinC function, which may explain why cell division inhibition by MinC(122-231) was not detected previously. These results suggest that the C-terminal half of MinC has an additional function in the regulation of Z-ring assembly.

Keywords

Adenosine Triphosphatases, Cell Division, Escherichia coli, Escherichia coli Proteins, Membrane Proteins, Protein Structure, Tertiary

Published Open-Access

yes

Recommended Citation

Daisuke Shiomi and William Margolin, "The CTSA University of Texas Health Science Center (UTHSC) Northeast—Tyler and Rio Grande Valley Success Story: How Rural, Underserved Academic Communities Rapidly Built a Robust Engine for Collaborative COVID-19 Clinical Research" (2007). Faculty, Staff and Student Publications. 163.
https://digitalcommons.library.tmc.edu/uthmed_docs/163