Faculty, Staff and Student Publications
Publication Date
2-1-2022
Journal
Nature Immunology
Abstract
Tuberculosis (TB) in humans is characterized by formation of immune-rich granulomas in infected tissues, the architecture and composition of which are thought to affect disease outcome. However, our understanding of the spatial relationships that control human granulomas is limited. Here, we used multiplexed ion beam imaging by time of flight (MIBI-TOF) to image 37 proteins in tissues from patients with active TB. We constructed a comprehensive atlas that maps 19 cell subsets across 8 spatial microenvironments. This atlas shows an IFN-γ-depleted microenvironment enriched for TGF-β, regulatory T cells and IDO1
Keywords
B7-H1 Antigen, Cells, Cultured, Cytokines, Gene Expression Profiling, Granuloma, Humans, Indoleamine-Pyrrole 2, 3, -Dioxygenase, Lung, Mycobacterium tuberculosis, Myeloid Cells, Tuberculosis
DOI
10.1038/s41590-021-01121-x
PMID
35058616
PMCID
PMC8810384
PubMedCentral® Posted Date
January 2022
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes