Faculty, Staff and Student Publications
Publication Date
5-12-2023
Journal
Science Advances
Abstract
CRISPR-Cas9 has been used successfully to introduce indels in somatic cells of rodents; however, precise editing of single nucleotides has been hampered by limitations of flexibility and efficiency. Here, we report technological modifications to the CRISPR-Cas9 vector system that now allows homology-directed repair-mediated precise editing of any proto-oncogene in murine somatic tissues to generate tumor models with high flexibility and efficiency. Somatic editing of either
Keywords
Animals, Mice, Humans, Gene Editing, CRISPR-Cas Systems, Neoplasms, Recombinational DNA Repair, Disease Models, Animal
DOI
10.1126/sciadv.ade0059
PMID
37172086
PMCID
PMC10181191
PubMedCentral® Posted Date
May 2023
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
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