Publication Date

5-12-2023

Journal

Science Advances

Abstract

CRISPR-Cas9 has been used successfully to introduce indels in somatic cells of rodents; however, precise editing of single nucleotides has been hampered by limitations of flexibility and efficiency. Here, we report technological modifications to the CRISPR-Cas9 vector system that now allows homology-directed repair-mediated precise editing of any proto-oncogene in murine somatic tissues to generate tumor models with high flexibility and efficiency. Somatic editing of either

Keywords

Animals, Mice, Humans, Gene Editing, CRISPR-Cas Systems, Neoplasms, Recombinational DNA Repair, Disease Models, Animal

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