Measurement of 8-Hydroxy-2′-Deoxyguanosine in Serum and Cerebrospinal Fluid of Horses With Neuroaxonal Degeneration and Other Causes of Proprioceptive Ataxia

Authors

Megan Palmisano
Jeaneen Kulp
Susan Bender
Darko Stefanovski
Mary Robinson
Amy Johnson

Publication Date

1-1-2024

Journal

Journal of Veterinary Internal Medicine

Abstract

BACKGROUND: Eight-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative damage evaluated in human neurodegenerative disease, has potential to correlate with postmortem diagnosis of neuroaxonal dystrophy/degenerative myeloencephalopathy (NAD/DM) in horses.

HYPOTHESIS: We hypothesized that 8-OHdG will be higher in CSF and serum from NAD/DM horses compared with horses with other neurologic diseases (CVSM, EPM) and a control group of neurologically normal horses. We also hypothesized that 8-OHdG will be higher in CSF compared with serum from NAD/DM horses.

ANIMALS: Fifty client-owned horses with postmortem diagnoses: 20 NAD/DM, 10 CVSM, 10 EPM, and 10 control horses. Serum and CSF samples were obtained between November 2010 and March 2022.

METHODS: Case-control study using biobanked samples was performed and commercial competitive ELISA kit (Highly Sensitive 8-OHdG Check ELISA) utilized. Concentration of 8-OHdG was quantitated in both CSF and serum and compared between groups.

RESULTS: No correlation was established between the measures of 8-OHdG in serum and CSF and group. CSF median [8-OHdG] for NAD/DM was 169.9 pg/mL (IQR

CONCLUSIONS: Eight-OHdG did not aid in antemortem diagnosis of NAD/DM in this cohort of horses. At the time of diagnosis horses with NAD/DM do not have ongoing oxidative stress.

Keywords

ataxia, equine degenerative myeloencephalopathy (EDM), equine neuroaxonal dystrophy (eNAD), neurodegenerative, oxidative damage

Comments

PMID: 38205913

Supplementary Materials