Faculty, Staff and Student Publications

Language

English

Publication Date

9-14-2022

Journal

Communications Biology

DOI

10.1038/s42003-022-03931-7

PMID

36104515

PMCID

PMC9474879

PubMedCentral® Posted Date

9-14-2022

PubMedCentral® Full Text Version

Post-print

Abstract

Natural killer (NK) cells mediate antibody dependent cytotoxic killing of cancer cells via cross-linking FcγR on NK cells with IgG-Fc. Studies have shown that the single-hinge cleaved IgGs (scIgGs) have dysfunctional Fc and failed engagement with FcγRs on immune cells. However, little is known about how scIgGs impact on antitumor immunity in the tumor microenvironment. In this study, we revealed a significant association of tumor scIgGs with tumor progression and poor outcomes of breast cancer patients (n = 547). Using multiple mouse tumor models, we demonstrated that tumor scIgGs reduced NK cell cytotoxic activities and resulted in aggressive tumor progression. We further showed that an anti-hinge specific monoclonal antibody (AHA) rescued the dysfunctional Fc in scIgGs by providing a functional Fc and restored NK cell cytotoxic activity. These findings point to a novel immunotherapeutic strategy to enhance Fc engagement with FcγRs for activation of anticancer immunity.

Keywords

Animals, Antineoplastic Agents, Immunoglobulin G, Killer Cells, Natural, Mice, Neoplasms, Neoplastic Processes, Tumor Microenvironment

Published Open-Access

yes

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