Rationale for reading fluconazole MICs at 24 hours rather than 48 hours when testing Candida spp by the CLSI M27-A2 standard method

Authors

Luis Ostrosky-Zeichner
John H Rex
Michael A Pfaller
Daniel J Diekema
Barbara D Alexander
David Andes
Steven D Brown
Vishnu Chaturvedi
Mahmoud A Ghannoum
Cindy C Knapp
Daniel J Sheehan
Thomas J Walsh

Publication Date

11-1-2008

Journal

Antimicrobial Agents and Chemotherapy

Abstract

We investigated if CLSI M27-A2 Candida species breakpoints for fluconazole MIC are valid when read at 24 h. Analysis of a data set showed good correlation between 48- and 24-h MICs, as well as similar outcomes and pharmacodynamic efficacy parameters, except for isolates in the susceptible dose-dependent category, such as Candida glabrata.

Keywords

Animals, Antifungal Agents, Candida, Candidiasis, Databases, Factual, Fluconazole, Humans, Microbial Sensitivity Tests, Models, Biological, Time Factors