Direct Visualization of The Putative Portal In The Kaposi's Sarcoma-Associated Herpesvirus Capsid By Cryoelectron Tomography

Authors

Binbin Deng
Christine M O'Connor
Dean H Kedes
Z Hong Zhou

Language

English

Publication Date

4-1-2007

Journal

Journal of Virology

DOI

10.1128/JVI.02254-06

PMID

17215290

PMCID

PMC1866054

PubMedCentral® Posted Date

January 2007

PubMedCentral® Full Text Version

Post-print

Abstract

Genetic and biochemical studies have suggested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses capsids, but its arrangement remained unknown. Here, we report the first visualization of a unique vertex in the Kaposi's sarcoma-associated herpesvirus (KSHV) capsid by cryoelectron tomography, thus providing direct structural evidence for the existence of a portal complex in a gammaherpesvirus. This putative KSHV portal is an internally localized, umbilicated structure and lacks all of the external machineries characteristic of portals in DNA bacteriophages.

Keywords

Capsid Proteins, Cryoelectron Microscopy, Herpesvirus 8, Human, Models, Molecular, Protein Structure, Quaternary

Published Open-Access

yes

Recommended Citation

Deng, Binbin; O'Connor, Christine M; Kedes, Dean H; et al., "Direct Visualization of The Putative Portal In The Kaposi's Sarcoma-Associated Herpesvirus Capsid By Cryoelectron Tomography" (2007). Faculty, Staff and Student Publications. 344.
https://digitalcommons.library.tmc.edu/uthmed_docs/344