Protocol for Non-invasive Pulsed-Doppler Evaluation of Blood Flow in the Mouse Ophthalmic Artery

Authors

• Radwa Awad
• Emil Martin
• Anilkumar K Reddy
• Iraida Sharina

Publication Date

9-19-2025

Journal

STAR Protocols

DOI

10.1016/j.xpro.2025.103893

PMID

40526471

PMCID

PMC12213287

PubMedCentral® Posted Date

6-16-2025

PubMedCentral® Full Text Version

Post-print

Abstract

The number of reliable and non-invasive techniques to characterize ocular blood flow is limited. Here, we present a protocol for identifying a soft tissue window in the orbital aperture using pulsed-Doppler ultrasonography to offer real-time, non-invasive blood flow velocity monitoring in the mouse ophthalmic artery. We describe steps for preparing a mouse, positioning the ultrasound probe, and acquiring and recording signals. We then detail procedures for analyzing results using a workstation equipped with Doppler flow velocity software. For complete details on the use and execution of this protocol, please refer to Sharina et al.

Keywords

Ultrasonography, Doppler, Pulsed, Animals, Mice, Blood Flow Velocity, Regional Blood Flow, Ophthalmic Artery, Models, Animal, Nitroprusside, Vasodilation, Mice, Inbred C57BL, Mice, Inbred BALB C, Male, Female, Health Sciences, Model Organisms, Molecular Biology

Published Open-Access

yes

Recommended Citation

Awad, Radwa; Martin, Emil; Reddy, Anilkumar K; et al., "Protocol for Non-invasive Pulsed-Doppler Evaluation of Blood Flow in the Mouse Ophthalmic Artery" (2025). Faculty, Staff and Student Publications. 3655.
https://digitalcommons.library.tmc.edu/uthmed_docs/3655