Airway Secretory Cells Contain Both a Perinuclear Golgi Ribbon and Dispersed Golgi Satellites

Authors

• Oanh N Hoang
• Colin E Chan
• Joshua M Brenner
• Denisse Leza-Rincon
• Ana M Jaramillo
• Brendan Dolan
• Adam W Aziz
• Rodolfo A Cardenas
• Gerardo J Cardenas
• Eduardo D Galvez
• Joshua B Hales
• Emilia S Nunez-Pena
• Boxuan Yang
• Reid T Powell
• Leoncio Vergara
• Harry Karmouty-Quintana
• Jesper M Magnusson
• Gunnar C Hansson
• Roberto Adachi
• John D Dickinson
• Christopher M Evans
• Justin A Courson
• Alan R Burns
• Michael J Tuvim
• Burton F Dickey

Language

English

Publication Date

2-21-2026

Journal

American Journal of Respiratory Cell and Molecular Biology

DOI

10.1093/ajrcmb/aanag018

PMID

41738292

Abstract

Rationale: Finely tuned production and secretion of the polymeric mucins MUC5AC and MUCB are required for lung health, but knowledge of many details between their translation and their packaging into secretory granules is lacking.

Objective: To analyze the structure and function of the Golgi apparatus, a key site of mucin glycosylation, folding, polymerization and packaging, in airway epithelial secretory cells.

Methods: Lung tissue was obtained from mice stimulated or not with IL-13 to upregulate mucin production, and from normal human lungs. Golgi elements in mouse and human tissue were imaged by high-resolution immunofluorescence microscopy and electron microscopy. Tissue from mice with deletion of both polymeric mucins was also examined.

Measurements and main results: By immunofluorescence microscopy, both mouse and human airway secretory cells contained ∼100 dispersed puncta labeled by markers of medial and trans Golgi cisternae and the trans-Golgi network (TGN), but only a few perinuclear puncta were labeled by markers of cis-Golgi cisternae. By electron microscopy, secretory cells contained both a perinuclear Golgi ribbon and numerous dispersed Golgi stacks, termed satellites. In mucous metaplastic cells, satellites were concentrated among immature mucin granules. Increasing mucin production by cytokine stimulation did not increase the number of TGN puncta, nor did preventing polymeric mucin production by gene deletion reduce TGN puncta.

Conclusions: Mucin-producing airway secretory cells express an unusual Golgi structure consisting of a conventional perinuclear ribbon as well as dispersed satellites. While the Golgi satellites are likely an adaptation for mucin production and packaging, their presence is specified developmentally, independent of mucin production.

Keywords

Golgi, MUC5AC, MUC5B, mucin, mucus

Published Open-Access

yes

Recommended Citation

Hoang, Oanh N; Chan, Colin E; Brenner, Joshua M; et al., "Airway Secretory Cells Contain Both a Perinuclear Golgi Ribbon and Dispersed Golgi Satellites" (2026). Faculty, Staff and Student Publications. 4075.
https://digitalcommons.library.tmc.edu/uthmed_docs/4075