MiR-9-1 Suppresses Cell Proliferation and Promotes Apoptosis by Targeting UHRF1 in Lung Cancer

Authors

Cheng-You Jia
Wei Xiang
Ji-Bin Liu
Geng-Xi Jiang
Feng Sun
Jian-Jun Wu
Xiao-Li Yang
Rui Xin
Yi Shi
Dan-Dan Zhang
Wen Li
Zavuga Zuberi
Jie Zhang
Gai-Xia Lu
Hui-Min Wang
Pei-Yao Wang
Fei Yu
Zhong-Wei Lv
Yu-Shui Ma
Da Fu

Language

English

Publication Date

1-1-2021

Journal

Technology in Cancer Research & Treatment

DOI

10.1177/15330338211041191

PMID

34520284

PMCID

PMC8445543

PubMedCentral® Posted Date

September 2021

PubMedCentral® Full Text Version

Post-print

Abstract

Lung cancer is listed as the most common reason for cancer-related death all over the world despite diagnostic improvements and the development of chemotherapy and targeted therapies. MicroRNAs control both physiological and pathological processes including development and cancer. A microRNA-9 to 1 (miR-9 to 1) overexpression model in lung cancer cell lines was established and miR-9 to 1 was found to significantly suppress the proliferation rate in lung cancer cell lines, colony formation in vitro, and tumorigenicity in nude mice of A549 cells. Ubiquitin-like containing PHD and RING finger domains 1 (UHRF1) was then identified to direct target of miR-9 to 1. The inhibition of UHRF1 by miR-9 to 1 causes G1 arrest and p15, p16, and p21 were re-expressed in miR-9 to 1 group in mRNA level and protein level. Silence of UHRF1 expression in A549 cells resulted in the similar re-expression of p15, p16, p21 which is similar with miR-9 to 1 infection. Therefore, we concluded that UHRF1 is a new target for miR-9 to 1 to suppress cell proliferation by re-expression of tumor suppressors p15, p16, and p21 mediated by UHRF1.

Keywords

Adult, Aged, Animals, Apoptosis, CCAAT-Enhancer-Binding Proteins, Cell Line, Tumor, Cell Movement, Cell Proliferation, Computational Biology, Disease Models, Animal, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Genes, Reporter, Heterografts, Humans, Lung Neoplasms, Male, Mice, MicroRNAs, Middle Aged, RNA Interference, Transcriptome, Ubiquitin-Protein Ligases

Published Open-Access

yes

Recommended Citation

Jia, Cheng-You; Xiang, Wei; Liu, Ji-Bin; et al., "MiR-9-1 Suppresses Cell Proliferation and Promotes Apoptosis by Targeting UHRF1 in Lung Cancer" (2021). Faculty, Staff and Student Publications. 628.
https://digitalcommons.library.tmc.edu/uthmed_docs/628