Faculty, Staff and Student Publications
Publication Date
1-1-2023
Journal
Antibody Therapeutics
Abstract
Acetaminophen (APAP) overdose is a leading cause of acute liver injury in the USA. The chitinase 3-like-1 (Chi3l1) protein contributes to APAP-induced liver injury (AILI) by promoting hepatic platelet recruitment. Here, we report the development of a Chi3l1-targeting antibody as a potential therapy for AILI. By immunizing a rabbit successively with the human and mouse Chi3l1 proteins, we isolated cross-reactive monoclonal antibodies (mAbs) from single memory B cells. One of the human and mouse Chi3l1 cross-reactive mAbs was humanized and characterized in both
Keywords
Chi3l1, acetaminophen, liver injury, antibody therapy, rabbit antibody, humanized antibody
DOI
10.1093/abt/tbac027
PMID
36683763
PMCID
PMC9847341
PubMedCentral® Posted Date
November 2022
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes