Journal Articles
Publication Date
1-31-2024
Journal
Viruses
Abstract
Rotaviruses (RVs) are a major cause of diarrhea in young children worldwide. The currently available and licensed vaccines contain live attenuated RVs. Optimization of live attenuated RV vaccines or developing non-replicating RV (e.g., mRNA) vaccines is crucial for reducing the morbidity and mortality from RV infections. Herein, a nucleoside-modified mRNA vaccine encapsulated in lipid nanoparticles (LNP) and encoding the VP7 protein from the G1 type of RV was developed. The 5' untranslated region of an isolated human RV was utilized for the mRNA vaccine. After undergoing quality inspection, the VP7-mRNA vaccine was injected by subcutaneous or intramuscular routes into mice. Mice received three injections in 21 d intervals. IgG antibodies, neutralizing antibodies, cellular immunity, and gene expression from peripheral blood mononuclear cells were evaluated. Significant differences in levels of IgG antibodies were not observed in groups with adjuvant but were observed in groups without adjuvant. The vaccine without adjuvant induced the highest antibody titers after intramuscular injection. The vaccine elicited a potent antiviral immune response characterized by antiviral clusters of differentiation CD8
Keywords
Child, Animals, Mice, Humans, Child, Preschool, Rotavirus, Rotavirus Vaccines, mRNA Vaccines, Rotavirus Infections, RNA, Messenger, CD8-Positive T-Lymphocytes, Leukocytes, Mononuclear, Antibodies, Viral, Capsid Proteins, Adjuvants, Immunologic, Vaccines, Attenuated, Immunoglobulin G
Comments
PMID: 38399987