Faculty, Staff and Student Publications
Publication Date
6-1-2022
Journal
Cancer Immunology, Immunotherapy
Abstract
An important mechanism of oncolytic virotherapy in ameliorating cancer immunotherapy is by inducing significant changes in the immune landscape in the tumor microenvironment (TME). Despite this notion and the potential therapeutic implications, a comprehensive analysis of the immune changes in carcinomas induced by virotherapy has not yet been elucidated. We conducted single-cell RNA sequencing analysis on carcinomas treated with an HSV-2-based oncolytic virus to characterize the immunogenic changes in the TME. We specifically analyzed and compared the immune cell composition between viral treated and untreated tumors. We also applied CellChat to analyze the complex interactions among the infiltrated immune cells. Our data revealed significant infiltration of B cells in addition to other important immune cells, including CD4
Keywords
Carcinoma, Humans, Immunotherapy, Oncolytic Virotherapy, Oncolytic Viruses, Sequence Analysis, RNA, Tumor Microenvironment, Single-cell RNA sequencing, Virotherapy, Oncolytic herpes simplex virus, Immune landscape, Tumor microenvironment
DOI
10.1007/s00262-021-03084-2
PMID
34716463
PMCID
PMC10992051
PubMedCentral® Posted Date
10-30-2021
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Included in
Bioinformatics Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Biomedical Informatics Commons, Immunotherapy Commons, Medical Immunology Commons, Oncology Commons
