Faculty, Staff and Student Publications

Language

English

Publication Date

4-6-2025

Journal

Nature Communications

DOI

10.1038/s41467-025-56989-2

PMID

40188094

PMCID

PMC11972378

PubMedCentral® Posted Date

4-6-2025

PubMedCentral® Full Text Version

Post-print

Abstract

The rapid growth of biomedical literature poses challenges for manual knowledge curation and synthesis. Biomedical Natural Language Processing (BioNLP) automates the process. While Large Language Models (LLMs) have shown promise in general domains, their effectiveness in BioNLP tasks remains unclear due to limited benchmarks and practical guidelines. We perform a systematic evaluation of four LLMs-GPT and LLaMA representatives-on 12 BioNLP benchmarks across six applications. We compare their zero-shot, few-shot, and fine-tuning performance with the traditional fine-tuning of BERT or BART models. We examine inconsistencies, missing information, hallucinations, and perform cost analysis. Here, we show that traditional fine-tuning outperforms zero- or few-shot LLMs in most tasks. However, closed-source LLMs like GPT-4 excel in reasoning-related tasks such as medical question answering. Open-source LLMs still require fine-tuning to close performance gaps. We find issues like missing information and hallucinations in LLM outputs. These results offer practical insights for applying LLMs in BioNLP.

Keywords

Natural Language Processing, Benchmarking, Humans, Large Language Models, Data mining, Health care

Published Open-Access

yes

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