Faculty, Staff and Student Publications

Language

English

Publication Date

7-3-2026

Journal

Briefings in Bioinformatics

DOI

10.1093/bib/bbag367

PMID

42430786

PMCID

PMC13354062

PubMedCentral® Posted Date

7-10-2026

PubMedCentral® Full Text Version

Post-print

Abstract

Large language models (LLMs) are deep learning-based artificial intelligence models that have achieved remarkable success in natural language processing. Typically composed of neural networks with billions of parameters, they are trained on massive unlabeled datasets using self-supervised or semi-supervised learning. Beyond language, LLMs hold immense potential for addressing complex bioinformatics challenges. This review provides a comprehensive overview of transformer-based model applications in genomics, transcriptomics, proteomics, drug discovery, and single-cell analysis. We discuss critical components, including tokenization strategies for diverse biological data, transformer architectures, attention mechanisms, and pretraining approaches. We also survey currently available foundation models and their downstream applications across bioinformatics domains. Finally, we highlight major challenges that remain insufficiently addressed in prior reviews and outline future perspectives and design principles for next-generation biological language models, offering practical guidance for both users and developers.

Keywords

Large Language Models, Computational Biology, Humans, Deep Learning, Genomics, Natural Language Processing, Proteomics, Drug Discovery, Neural Networks, Computer, Single-Cell Analysis, language model, foundation model, transformer architecture, multi-omics application, drug discovery, single-cell analysis

Published Open-Access

yes

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