A Multi-Level Gene-Diet Interaction Analysis of Fish Oil and 14 Polyunsaturated Fatty Acid Traits Identifies the FADS and GPR12 Loci

Authors

Susan Adanna Ihejirika
Alexandra Huong Chiang
Aryaman Singh
Eunice Stephen
Han Chen
Kaixiong Ye

Language

English

Publication Date

5-21-2025

Journal

Human Genetics and Genomics Advances

DOI

10.1016/j.xhgg.2025.100459

PMID

40405463

PMCID

PMC12172259

PubMedCentral® Posted Date

5-21-2025

PubMedCentral® Full Text Version

Post-print

Abstract

Fish oil supplements (FOS) are known to alter circulating levels of polyunsaturated fatty acids (PUFAs) but in a heterogeneous manner across individuals. These varied responses may result from unidentified gene-FOS interactions. To identify genetic factors that interact with FOS to alter the circulating levels of PUFAs, we performed a multi-level genome-wide interaction study (GWIS) of FOS on 14 plasma measurements in 200,060 unrelated European-ancestry individuals from the UK Biobank. From our single-variant tests, we identified genome-wide significant interacting SNPs (p < 5 × 10-8) in the FADS1-FADS2 gene cluster for total omega-3, omega-3%, docosapentaenoic acid (DHA), DHA%, and the omega-6 to omega-3 ratio. Among the interaction signals for omega-3%, the lead SNP, rs35473591 (C>CT, CT allele frequency = 0.34), had a lower association effect size in the FOS-taking group (β = 0.35 for allele C) than that in the group without FOS (β = 0.42). Likewise, the effect sizes of associations between FOS and omega-3% varied across the three genotype groups (β = 0.45, 0.50, and 0.59, respectively, in C/C, C/CT, and CT/CT). Our gene-level aggregate and transcriptome-wide interaction analyses identified significant signals at two loci around FADS1-FADS2 and GPR12. The contribution of genome-wide gene-FOS interactions to phenotypic variance was statistically significant in omega-3-related traits. This systematic gene-FOS GWIS contributes to our understanding of the genetic architecture of circulating PUFAs underlying FOS response and informs personalized dietary recommendations.

Keywords

gene-diet interactions, polyunsaturated fatty acids, fish oil supplements, omega-3

Published Open-Access

yes

Recommended Citation

Ihejirika, Susan Adanna; Chiang, Alexandra Huong; Singh, Aryaman; et al., "A Multi-Level Gene-Diet Interaction Analysis of Fish Oil and 14 Polyunsaturated Fatty Acid Traits Identifies the FADS and GPR12 Loci" (2025). Faculty, Staff and Student Publications. 1073.
https://digitalcommons.library.tmc.edu/uthsph_docs/1073