Faculty, Staff and Student Publications
Publication Date
4-15-2024
Journal
World Journal of Diabetes
Abstract
BACKGROUND: Icariin (ICA), a natural flavonoid compound monomer, has multiple pharmacological activities. However, its effect on bone defect in the context of type 1 diabetes mellitus (T1DM) has not yet been examined.
AIM: to explore the role and potential mechanism of ICA on bone defect in the context of T1DM.
METHODS: The effects of ICA on osteogenesis and angiogenesis were evaluated by alkaline phosphatase staining, alizarin red S staining, quantitative real-time polymerase chain reaction, Western blot, and immunofluorescence. Angiogenesis-related assays were conducted to investigate the relationship between osteogenesis and angiogenesis. A bone defect model was established in T1DM rats. The model rats were then treated with ICA or placebo and micron-scale computed tomography, histomorphometry, histology, and sequential fluorescent labeling were used to evaluate the effect of ICA on bone formation in the defect area.
RESULTS: ICA promoted bone marrow mesenchymal stem cell (BMSC) proliferation and osteogenic differentiation. The ICA treated-BMSCs showed higher expression levels of osteogenesis-related markers (alkaline phosphatase and osteocalcin) and angiogenesis-related markers (vascular endothelial growth factor A and platelet endothelial cell adhesion molecule 1) compared to the untreated group. ICA was also found to induce osteogenesis-angiogenesis coupling of BMSCs. In the bone defect model T1DM rats, ICA facilitated bone formation and CD31
CONCLUSION: ICA was able to accelerate bone regeneration in a T1DM rat model by inducing osteogenesis-angiogenesis coupling of BMSCs.
Keywords
Icariin, Osteogenesis-angiogenesis coupling, Type 1 diabetes mellitus, Bone defect, Bone regeneration
DOI
10.4239/wjd.v15.i4.769
PMID
38680705
PMCID
PMC11045423
PubMedCentral® Posted Date
April 2024
PubMedCentral® Full Text Version
Post-print
Included in
Endocrinology, Diabetes, and Metabolism Commons, Internal Medicine Commons, Public Health Commons