Faculty, Staff and Student Publications
Publication Date
3-21-2024
Journal
Stem Cell Research
Abstract
Somatostatin (SST)-producing pancreatic delta-cells play an important role in maintaining the balance of insulin and glucagon secretion within the islets. This study aimed to generate a human embryonic stem cell (hESC) line with a SST-P2A-mCherry reporter using CRISPR/Cas9 system. The SST-P2A-mCherry reporter cell line was shown to maintain typical pluripotent characteristics and able to be induced into SST-producing pancreatic delta-cells. The generation of the cell line would provide useful platform for the characterization of stem cell-derived delta-cells, discovery of delta-cell surface markers and investigation of paracrine mechanisms, which will ultimately promote the drug discovery and cell therapy of diabetes mellitus.
DOI
10.1016/j.scr.2024.103397
PMID
38547668
Included in
Endocrine System Diseases Commons, Endocrinology, Diabetes, and Metabolism Commons, Public Health Commons