Generation of Sst-P2a-Mcherry Reporter Human Embryonic Stem Cell Line Using the Crispr/cas9 System (WAe001-A-2C)

Authors

Tongran Zhang
Feng Zhang
Nannan Wang
Tao Xu
Lingqiang Zhu
Lihua Chen
Huisheng Liu

Publication Date

3-21-2024

Journal

Stem Cell Research

Abstract

Somatostatin (SST)-producing pancreatic delta-cells play an important role in maintaining the balance of insulin and glucagon secretion within the islets. This study aimed to generate a human embryonic stem cell (hESC) line with a SST-P2A-mCherry reporter using CRISPR/Cas9 system. The SST-P2A-mCherry reporter cell line was shown to maintain typical pluripotent characteristics and able to be induced into SST-producing pancreatic delta-cells. The generation of the cell line would provide useful platform for the characterization of stem cell-derived delta-cells, discovery of delta-cell surface markers and investigation of paracrine mechanisms, which will ultimately promote the drug discovery and cell therapy of diabetes mellitus.