Publication Date
6-17-2022
Journal
STAR Protocols
DOI
10.1016/j.xpro.2022.101392
PMID
35600933
PMCID
PMC9120230
PubMedCentral® Posted Date
5-17-2022
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Animals, Animals, Newborn, Fatty Acids, Mice, Myocytes, Cardiac, Respiration, Smegmamorpha, Cell Biology, Cell culture, Cell isolation, Metabolism, Protein Biochemistry
Abstract
Metabolic switches play a critical role in the pathophysiology of cardiac diseases, including heart failure. Here, we describe an assay for long-chain fatty acid oxidation in neonatal mouse cardiomyocytes by using a SeaHorse Flux Analyzer (Agilent). This protocol is a simplified but robust adaptation of the standard protocol that enables metabolic measurements in cells isolated from transgenic mouse models, which can be timesaving and informative. Cell isolation and culture represent a critical point that may require bench optimization.
For complete details on the use and execution of this protocol, please refer to Angelini et al. (2021).
Graphical Abstract
Included in
Biochemical Phenomena, Metabolism, and Nutrition Commons, Cardiology Commons, Cardiovascular Diseases Commons, Medical Biochemistry Commons, Medical Cell Biology Commons
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