Language
English
Publication Date
10-20-2022
Journal
Molecular Cell
DOI
10.1016/j.molcel.2022.08.027
PMID
36113481
PMCID
PMC9588704
PubMedCentral® Posted Date
10-20-2020
PubMedCentral® Full Text Version
Author MSS
Abstract
Ribosomal RNAs (rRNAs) are the most abundant cellular RNAs, and their synthesis from rDNA repeats by RNA polymerase I accounts for the bulk of all transcription. Despite substantial variation in rRNA transcription rates across cell types, little is known about cell-type-specific factors that bind rDNA and regulate rRNA transcription to meet tissue-specific needs. Using hematopoiesis as a model system, we mapped about 2,200 ChIP-seq datasets for 250 transcription factors (TFs) and chromatin proteins to human and mouse rDNA and identified robust binding of multiple TF families to canonical TF motifs on rDNA. Using a 47S-FISH-Flow assay developed for nascent rRNA quantification, we demonstrated that targeted degradation of C/EBP alpha (CEBPA), a critical hematopoietic TF with conserved rDNA binding, caused rapid reduction in rRNA transcription due to reduced RNA Pol I occupancy. Our work identifies numerous potential rRNA regulators and provides a template for dissection of TF roles in rRNA transcription.
Keywords
Humans, Mice, Animals, RNA Polymerase I, Transcription Factors, RNA, Ribosomal, Transcription, Genetic, DNA, Ribosomal, RNA, Chromatin
Published Open-Access
yes
Recommended Citation
Antony, Charles; George, Subin S; Blum, Justin; et al., "Control of Ribosomal RNA Synthesis by Hematopoietic Transcription Factors" (2022). Faculty and Staff Publications. 373.
https://digitalcommons.library.tmc.edu/baylor_docs/373
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Biology Commons, Medical Sciences Commons, Medical Specialties Commons