Gut Microbial Protein Expression in Response to Dietary Patterns in a Controlled Feeding Study: A Metaproteomic Approach

Authors

Sheng Pan
Meredith A J Hullar
Lisa A Lai
Hong Peng
Damon H May
William S Noble
Daniel Raftery
Sandi L Navarro
Marian L Neuhouser
Paul D Lampe
Johanna W Lampe
Ru Chen

Publication Date

3-7-2020

Journal

Microorganisms

DOI

10.3390/microorganisms8030379

PMID

32156071

PMCID

PMC7143255

PubMedCentral® Posted Date

3-7-2020

PubMedCentral® Full Text Version

Post-Print

Published Open-Access

yes

Keywords

Microbiome, proteomics, dietary intervention, SCFA, fatty acid metabolism, dysbiosis, mucin-degrading enzymes

Abstract

Although the gut microbiome has been associated with dietary patterns linked to health, microbial metabolism is not well characterized. This ancillary study was a proof of principle analysis for a novel application of metaproteomics to study microbial protein expression in a controlled dietary intervention. We measured the response of the microbiome to diet in a randomized crossover dietary intervention of a whole-grain, low glycemic load diet (WG) and a refined-grain, high glycemic load diet (RG). Total proteins in stools from 9 participants at the end of each diet period (

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