Dimethyl Sulfoxide Inhibits Bile Acid Synthesis in Healthy Mice but Does Not Protect Mice from Bile-Acid-Induced Liver Damage

Authors

Xi Chen
Huiqiao Li
Yu'e Liu
Jing Qi
Bingning Dong
Shixia Huang
Shangang Zhao
Yi Zhu

Publication Date

8-9-2023

Journal

Biology

DOI

10.3390/biology12081105

PMID

37626991

PMCID

PMC10452260

PubMedCentral® Posted Date

8-9-2023

PubMedCentral® Full Text Version

Post-Print

Published Open-Access

yes

Keywords

dimethyl sulfoxide (DMSO), bile acid, liver damage, nonalcoholic steatohepatitis (NASH)

Abstract

Bile acids serve a vital function in lipid digestion and absorption; however, their accumulation can precipitate liver damage. In our study, we probed the effects of dimethyl sulfoxide (DMSO) on bile acid synthesis and the ensuing liver damage in mice induced by bile acids. Our findings indicate that DMSO efficaciously curbs bile acid synthesis by inhibiting key enzymes involved in the biosynthetic pathway, both in cultured primary hepatocytes and in vivo. Contrarily, we observed that DMSO treatment did not confer protection against bile-acid-induced liver damage in two distinct mouse models: one induced by a 0.1% DDC diet, leading to bile duct obstruction, and another induced by a CDA-HFD, resulting in non-alcoholic steatohepatitis (NASH). Histopathological and biochemical analyses unveiled a comparable extent of liver injury and fibrosis levels in DMSO-treated mice, characterized by similar levels of increase in

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