Publication Date
10-1-2023
Journal
Advanced Healthcare Materials
DOI
10.1002/adhm.202300960
PMID
37395729
PMCID
PMC10592251
PubMedCentral® Posted Date
10-1-2024
PubMedCentral® Full Text Version
Author MSS
Published Open-Access
yes
Keywords
Rats, Animals, Hyperplasia, Rats, Sprague-Dawley, Arteriovenous Shunt, Surgical, Neointima, Absorbable Implants, Tomography, X-Ray Computed, Renal Insufficiency, Chronic, Arteriovenous Fistula, Mesenchymal Stem Cells, Tissue Scaffolds, arteriovenous fistula, end-stage renal disease, mesenchymal stem cell, polymer, positron emission tomography, ultrasonography
Abstract
Bioresorbable perivascular scaffolds loaded with antiproliferative agents have been shown to enhance arteriovenous fistula (AVF) maturation by inhibiting neointimal hyperplasia (NIH). These scaffolds, which can mimic the three-dimensional architecture of the vascular extracellular matrix, also have an untapped potential for the local delivery of cell therapies against NIH. Hence, an electrospun perivascular scaffold from polycaprolactone (PCL) to support mesenchymal stem cell (MSC) attachment and gradual elution at the AVF's outflow vein is fabricated. Chronic kidney disease (CKD) in Sprague-Dawley rats is induced by performing 5/6th nephrectomy, then AVFs for scaffold application are created. The following groups of CKD rats are compared: no perivascular scaffold (i.e., control), PCL alone, and PCL+MSC scaffold. PCL and PCL+MSC significantly improve ultrasonographic (i.e., luminal diameter, wall-to-lumen ratio, and flow rate) and histologic (i.e., neointima-to-lumen ratio, neointima-to-media ratio) parameters compared to control, with PCL+MSC demonstrating further improvement in these parameters compared to PCL alone. Moreover, only PCL+MSC significantly reduces
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Diseases Commons, Internal Medicine Commons, Medical Sciences Commons, Nephrology Commons
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