Publication Date
6-1-2022
Journal
Phage
DOI
10.1089/phage.2022.0004
PMID
36157281
PMCID
PMC9248872
PubMedCentral® Posted Date
6-16-2022
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
virulent, lytic, pseudintermedius, canine, pyoderma
Abstract
Background:
There are no verified lytic Staphylococcus pseudintermedius phages in the literature and few temperate phage genomes in databases. S. pseudintermedius is an opportunistic zoonotic pathogen of great importance in veterinary and human medicine.
Materials and Methods:
We discovered phages against canine-derived S. pseudintermedius isolates by screening dog feces, hair, and skin swabs. Fourteen new phages were isolated and characterized by genomic analysis, transmission electron microscopy, and host range determination.
Results:
Three phages—DH2, DH5, and DS10, a phage K variant—were predicted lytic by sequencing, a designation supported by mitomycin C induction. All three are S. pseudintermedius and Staphylococcus aureus polyvalent phages, with DH2 and DS10 being strong killers of both species.
Conclusions:
We report discovery of the first verified lytic S. pseudintermedius phages and suggest dog hair as a novel reservoir. DH2, DH5, and DS10 are promising candidates toward developing an anti-Staphylococcal phage cocktail.