Publication Date
9-26-2024
Journal
International Journal of Molecular Sciences
DOI
10.3390/ijms251910333
PMID
39408663
PMCID
PMC11476625
PubMedCentral® Posted Date
9-26-2024
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Female, Humans, Pregnancy, Biomarkers, Metabolomics, Adult, Pregnancy, Ectopic, Case-Control Studies, Metabolome, Tandem Mass Spectrometry, Chromatography, Liquid, Prospective Studies, ectopic pregnancy, gynecology, metabolomics, molecular networking, untargeted, biomarker, metabolite
Abstract
Ectopic pregnancy (EP) is the leading cause of maternal morbidity and mortality in the first trimester. Using an untargeted metabolomic approach, we sought to identify putative plasma biomarkers using tandem liquid chromatography-mass spectrometry for the detection of tubal EP. This case-control study included the prospective recruitment of 50 tubal EP cases and 50 early intrauterine pregnancy controls. To avoid over-fitting, logistic regression models were developed in a randomly selected discovery group (30 cases vs. 30 controls) and validated in the test group (20 cases vs. 20 controls). In total, 585 mass spectral features were detected, of which 221 molecular features were significantly altered in EP plasma (