Language
English
Publication Date
2-14-2025
Journal
Scientific Data
DOI
10.1038/s41597-025-04528-5
PMID
39952952
PMCID
PMC11829053
PubMedCentral® Posted Date
2-14-2025
PubMedCentral® Full Text Version
Post-print
Abstract
The trabecular meshwork within the outflow apparatus is critical in maintaining intraocular pressure homeostasis. In vitro studies employing primary cell cultures of the human trabecular meshwork (hTM) have conventionally served as surrogates for investigating the pathobiology of TM dysfunction. Despite its abundant use, translation of outcomes from in vitro studies to ex vivo and/or in vivo studies remains a challenge. Given the cell heterogeneity, performing single-cell RNA sequencing comparing primary hTM cell cultures to hTM tissue may provide important insights on cellular identity and translatability, as such an approach has not been reported before. In this study, we assembled a total of 14 primary hTM in vitro samples across passages 1-4, including 4 samples from individuals diagnosed with glaucoma. This dataset offers a comprehensive transcriptomic resource of primary hTM in vitro scRNA-seq data to study global changes in gene expression in comparison to cells in tissue in situ. We have performed extensive preprocessing and quality control, allowing the research community to access and utilize this public resource.
Keywords
Trabecular Meshwork, Humans, Single-Cell Analysis, Transcriptome, Glaucoma, Cells, Cultured, Sequence Analysis, RNA, Biomarkers, Data integration, Transcriptomics
Published Open-Access
yes
Recommended Citation
Tian, Alice; Kim, Sangbae; Baidouri, Hasna; et al., "Divergence in Cellular Markers Observed in Single-Cell Transcriptomics Datasets Between Cultured Primary Trabecular Meshwork Cells and Tissues" (2025). Faculty and Staff Publications. 4351.
https://digitalcommons.library.tmc.edu/baylor_docs/4351