Language
English
Publication Date
7-11-2025
Journal
The Journal of Infectious Diseases
DOI
10.1093/infdis/jiaf123
PMID
40048623
PMCID
PMC12247812
PubMedCentral® Posted Date
3-6-2025
PubMedCentral® Full Text Version
Post-print
Abstract
The diagnosis of chronic Chagas disease is challenging due to the wide genetic diversity of Trypanosoma cruzi, the causative agent of Chagas disease, and low levels of parasitemia, resulting in low sensitivity and accuracy with existing diagnostics. We report a magneto-immunoassay that employs dually labeled magnetic beads incorporating a recombinant Tc24 antigen, which is homologous across multiple discrete typing units of T cruzi. In this pilot study, 102 serum samples from 7 endemic countries were tested by this magneto-immunoassay, revealing its ability to distinguish Chagas-positive from Chagas-negative cases more accurately and faster than a standard Tc24-based immunoassay.
Keywords
Chagas Disease, Pilot Projects, Trypanosoma cruzi, Humans, Antigens, Protozoan, Antibodies, Protozoan, Immunoassay, Sensitivity and Specificity, Recombinant Proteins, Chagas disease, diagnostic, immunoassay, Tc24, Trypanosoma cruzi
Published Open-Access
yes
Recommended Citation
Singampalli, Kavya L; Zhan, Bin; Mejia, Rojelio; et al., "Rapid High-Sensitivity Detection of Antibodies to Trypanosoma cruzi With a Recombinant Tc24 Antigen-Based Magneto-Immunoassay: A Pilot Study" (2025). Faculty and Staff Publications. 4711.
https://digitalcommons.library.tmc.edu/baylor_docs/4711